1. Analysis of biological samples and chemical analysis of environmental pollutants using separation techniques, including:

high-performance liquid chromatography (HPLC-MS/MS; HPLC-UV-Vis DAD; HPLC-FLD; HPLC-LSD), gas chromatography (GC-MS), capillary electrophoresis (CE-UV-Vis DAD).

2. Chemistry and biochemistry of thiol amino acids, homocysteine ​​thiolactone and their metabolites.

3. Development of analytical methods enabling the determination of selected biologically important compounds in complex matrices.

4. Preparation of a biological sample for analysis; chemical derivatization.

5. Study of the possibilities of increasing sensitivity in capillary electrophoresis using concentration of analytes in the capillary.

Some publications:

1. A. Undas, J. Perła‑Kaján, R. Głowacki, Homocysteine in adult patients with cardiovascular disease: is it clinically relevant in 2025? A tribute to Hieronim Jakubowski, (1946–2025), Polish Archives of Internal Medicine, 135 ( 2025) 1-8.

2. A. Krata, J. Domagała, R. Głowacki, Hydrophilic interaction liquid chromatography based method for simultaneous determination of purines and their derivatives in food spices, Food Chemistry, 441 (2024) 138285.

3. J. Piechocka, R. Głowacki, Comprehensive studies on the development of HPLC-MS/MS and HPLC-FL based methods for routine determination of homocysteine thiolactone in human urine, Talanta, 272 (2024) 125791.

4. J. Piechocka, N. Matwiej, M. Gaweł, M. Matyjaszczyk, R. Głowacki, G. Chwatko, Application of the HPLC-ELSD technique for the determination of major metabolites of ibuprofen and creatinine in human urine, Scientific Reports, 13 (2023) 20268.

5. J. Piechocka, R. Głowacki, One-pot sample preparation procedure for the determination of protein N-linked homocysteine by HPLC-FLD based method, J. Chromatogr. B, 1228 (2023) 123835.

6. J. Piechocka, M. Wyszczelska-Rokiel, R. Głowacki, Simultaneous determination of 2-(3-hydroxy-5-phosphonooxymethyl-2-methyl-4-pyridyl)-1,3-thiazolidine-4-carboxylic acid and main plasma aminothiols by HPLC-UV based method, Scientific Reports, 13 (2023) 9294, 1-12.

7. J. Piechocka, R. Głowacki, Up-to-date knowledge about analytical methods for homocysteine thiolactone determination in biological samples, Trends Anal. Chem. – TrAC, 159 (2023) 116906.

8. R. Głowacki, J. Piechocka, E. Bald, G. Chwatko, Application of Separation Techniques in Analytics of Biologically Relevant Sulfur Compounds, in Handbook of Bioanalytics (B. Buszewski, I. Baranowska (eds.)), Springer Nature, 2022, 233-256.

9. Bekier, J. Gatkowska, M. Chyb, J. Sokołowska, G. Chwatko, R. Głowacki, A. Paneth, K. Dzitko, 4-Arylthiosemicarbazide derivatives – pharmacokinetics, toxicity and anti-Toxoplasma gondii activity in vivo, Eur. J. Med. Chem. 244 (2022) 114812.

10. J. Piechocka, M. Wieczorek, R. Głowacki, Gas Chromatography – Mass Spectrometry Based Approach For the Determination of Methionine – Related Sulfur Containing Compounds In Human Saliva, Int. J. Mol. Sci. 21, 2020, 9252.

11. J. Piechocka, M. Wrońska, R. Głowacki, Chromatographic strategies for the determination of aminothiols in human saliva, Trends Anal. Chem. – TrAC, 126 (2020) 115866.

12. J. Piechocka, M. Wrońska, G. Chwatko, H. Jakubowski, R. Głowacki, Quantification of homocysteine thiolactone in human saliva and urine by gas chromatography-mass spectrometry, J. Chromatogr. B. 1149 (2020) 122155.

13. J. Piechocka, M. Wrońska, I.E. Głowacka, R. Głowacki, 2-(3-Hydroxy-5-phosphonooxymethyl-2-methyl-4-pyridyl)-1,3-thiazolidine-4-carboxylic Acid, Novel Metabolite of Pyridoxal 5’-Phosphate and Cysteine Is Present in Human Plasma - Chromatographic Investigations, Int. J. Mol. Sci. 21 (2020) 3548.

14. K. Morawska, N. Festinger, G. Chwatko, R. Głowacki, W. Ciesielski, S. Smarzewska, Rapid electroanalytical procedure for sesamol determination in real samples, Food Chem. 309 (2020) 125789.

15. P. Kubalczyk K. Purgat, P. Olejarz, I. Kośka, R. Głowacki, Determination of homocysteine thiolactone in human urine by capillary zone electrophoresis and single drop microextraction, Anal. Biochem., 596 (2020) 113640.

16. R.M. Dyson, H.K. Palliser, N. Wilding, M.A. Kelly, G. Chwatko, R. Głowacki, M.J. Berry, X. Ni, I.M.R Wright, Microvascular circulatory dysregulation driven in part by cystathionine-gamma-lyase: a new paradigm for cardiovascular compromise in the preterm newborn, Microcirculation, (2019) 26: e12507.

17. K. Borowczyk, J. Piechocka, R. Głowacki, I. Dhar, Ø. Midtun, G.S. Tell, P.M. Ueland, O. Nygård, H. Jakubowski, Urinary Excretion of Homocysteine Thiolactone and the Risk of Acute Myocardial Infarction in Coronary Artery Disease Patients; the Western Norway B-Vitamin Intervention Trial, J. Int. Med., 285 (2019) 232-244.

18. K. Purgat, K. Borowczyk, R. Zakrzewski, R. Głowacki, P. Kubalczyk, Determination of nikethamide by micellar electrokinetic chromatography, Biomed. Chromatogr. 2019;33:e4571.

19. Kamińska, I.E. Głowacka, B. Pasternak, R. Głowacki, G. Chwatko, New method for simultaneous determination of lipoyllysine and α-lipoic acid by high performance liquid chromatography, Bioanalysis, 11 (2019) 1359-1373.

20. G. Chwatko, M. Krawczyk, M. Iciek, A. Kamińska, A. Bilska-Wilkosz, B. Marcykiewicz, R. Głowacki, Determination of lipoic acid in human plasma by high-performance liquid chromatography with ultraviolet detection, Arab. J. Chem., 12 (2019) 4878–4886.

21. J. Perła-Kajan, K. Borowczyk, R. Głowacki, O. Nygård, H. Jakubowski, Paraoxonase 1 (PON1) genotype and activity affect homocysteine thiolactone levels in humans, FASEB Journal, 32 (2018) 6019-6024.

22. M. Wrońska, G. Chwatko, K. Borowczyk, J. Piechocka, P. Kubalczyk, R. Głowacki, Application of GC-MS technique for the determination of homocysteine thiolactone in human urine, J. Chromatogr. B, 1099 (2018) 18–24. P.